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EMBO Member

Karla Neugebauer

Yale University, New Haven | United States

EMBO 2011

Transcription and RNA processing in living cells

We have established experimental systems in budding yeast, zebrafish embryos, and mammalian tissue culture cells to explore transcription and splicing regulation in a variety of biological contexts and with diverse tools, from imaging to genome-wide approaches. Recent discoveries include 1) terminal exon pausing, in which RNA polymerase II pauses before gene ends to permit co-transcriptional splicing and 2) the essential role of the Cajal body in zebrafish embryogenesis; the CB supports high levels of splicing by promoting spliceosomal snRNP assembly.

Keywords: Pre-mRNA splicing / ribonucleoproteins / nuclear organization & dynamics / Cajal bodies / transcription

Subject area(s): Chromatin & Transcription | Genomic & Computational Biology | RNA

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