Control of tRNA biosynthesis in yeast
My group is interested in mechanism of tRNA transcription by RNA polymerase III and its control by external conditions. This control is mediated by Maf1, a global negative regulator conserved in eukaryotes. Yeast Maf1 was originally found in our laboratory and subsequently we established the mode of Maf1 activation in response to stress. Recently we have shown unequal distribution of polymerase along tDNA genes and substantial readthrough of termination signal typically extending 50–200 nt.
Keywords
tRNA / RNA polymerase / Maf1 / transcription mechanism / yeast