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EMBO Member

Marie-France Carlier

Gif-sur-Yvette | France

EMBO 2001

Regulation of actin assembly in cell motility

I use bulk solution and single filament kinetics and thermodynamic analysis of actin filament assembly in vitro to understand the molecular mechanisms by which polarized actin assembly is regulated in motility. I characterize the function of all main regulators of actin assembly. My goal is to challenge the derived mechanistic insight to reconstitute motile processes in vitro with a minimal number of pure components.

Keywords: Cytoskeleton dynamics / cell motility / actin self-assembly / actin regulatory proteins / Arp2/3 complex / formins

Subject area(s): Cell & Tissue Architecture | Proteins & Biochemistry | Structural Biology & Biophysics

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