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EMBO Associate Member

Philip C. Hanawalt

Stanford University | United States

EMBO 2001

Transcription blockage in unusual DNA sequences

We study RNA polymerase arrest at lesions and in unusual DNA sequences, to learn mechanisms of transcription-coupled DNA repair (TCR). Cockayne syndrome (CS) and UV-sensitive syndrome are TCR deficient and present no cancers, but CS cells are sensitive to reactive oxygen species. We have developed an ultrasensitive comet-FISH assay to measure oxidative damage and its repair in expressed genes.

Keywords: DNA repair / DNA replication / transcription / human genetic diseases / environmental stress responses

Subject area(s): Chromatin & Transcription | Genome Stability & Dynamics | Molecular Medicine

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