We shifted a number of years ago from studying cytotoxicity in mouse to studying cell death first in mouse then in Dictyostelium discoideum. In the latter, cell death occurs during starvation-induced development, but can be studied in cell monolayers. Double-signaling by starvation then in particular by the differentiation factor DIF-1 leads to death with vacuolization and cellulose walls. The molecular bases of these events are being studied using random insertional mutagenesis.
Keywords: Cell death / molecular mechanisms / Dictyostelium